Microbiology (Main)

Background

• Culture and gram stain results

Gram Negatives

1. Cocci
   1. Neisseria
      1. meningitidis (meningococcus)
      2. gonorrhoeae (gonococcus)
   2. Moraxella
2. Rods
   1. Lactose Fermenters
   2. Non-Lactose Fermenters

Lactose Fermenters

Lactose Fermenters	Indole	morphology
E. Coli	+	flat
Klebsiella	-	muccoid
Enterobacter	-	muccoid
Citrobacter	-	flat
Arizona	-

Non-Lactose Fermenters

Non-Lactose Fermenters	Characteristics
Pseudomonas	usually strict aerobes, oxidase +, small
Salmonella, Shigella
Acinetobacter	coccobac
Proteus	swarming
H. Flu	tiny, chocolate agar
Pasteurella

Gram Positives

1. Cocci
   1. Clusters
      1. catalase + Staph sp
      2. coagulase + S. aureus
      3. coagulase - S. epidermidis
   2. Pairs/chains -
      1. catalase - Strep sp.
2. Rods

Rods

Rods		Cult	Morphology
Listeria	Small	Aero	tumbling
Diphtheroids	Small	Anaero	pallisades
Actinomyces	Small	Anaero	Branching
Propionibac	Small	Anaero	clumps/pleo
Lactobacillus	Variable	Both	may chain
Clostridium	Large	Anaero	Spores
Bacillus	Large	Aero	Spores

Anaerobes

1. Origin - gut, mouth, head/neck (50% of chronic sinusitis), lung, female gu tract
2. Cant grow unless culture specifically asked. 3% of bacteremia, <1% of cystitis
3. Bacteroides (B.frag GNR) (Peptostreptococus GPC) (P. Acnes GPR), Clostridium GPR)

Source

7/2/09 PANI