Microbiology (Main)

I. Gram Negatives

A. Cocci

• Neisseria sp. meningococcus, Moraxella

B.� Rods

1) Lactose Fermenters	Indole���	morphology
E. Coli	+	flat
Klebsiella	-	muccoid
Enterobacter	-	muccoid
Citrobacter	-	flat
Arizona	-

2). Non-Lactose Fermenter	Characteristics ��
Pseudomonas	usually strict aerobes, oxidase +, small
Salmonella, Shigella
Acinetobacter	coccobac
Proteus	swarming
H. Flu	tiny, chocolate agar
Pasteurella

II. Gram Positives

A.� Cocci

1)� Clusters

i. catalase + Staph sp

ii. coagulase +�� S. aureus

iii. coagulase -�� S. epidermidis

2)� Pairs/chains -

i. catalase -� Strep sp.

B. Rods		Cult	Morphology
Listeria	Small	Aero	tumbling
Diphtheroids	Small	Anaero	pallisades
Actinomyces	Small	Anaero	Branching
Propionibac	Small	Anaero	clumps/pleo
Lactobacillus	Variable	Both	may chain
Clostridium	Large	Anaero	Spores
Bacillus	Large	Aero	Spores

III. Anaerobes

1. Origin - gut, mouth, head/neck (50% of chronic sinusitis), lung, female gu tract

2. Cant grow unless culture specifically asked. 3% of bacteremia, <1% of cystitis

3. Bacteroides (B.frag GNR) (Peptostreptococus GPC) (P. Acnes GPR), Clostridium GPR)

7/2/09 PANI

I. Gram Negatives

A. Cocci

• Neisseria sp. meningococcus, Moraxella

B. Rods

1) Lactose Fermenters Indole morphology

E.  Coli      

+ flat

Klebsiella	-	muccoid 	
Enterobacter	- 	muccoid	

Citrobacter - flat Arizona -

2). Non-Lactose Fermenter Characteristics Pseudomonas

usually strict aerobes, oxidase +, small Salmonella, Shigella Acinetobacter coccobac Proteus swarming H. Flu tiny, chocolate agar Pasteurella

II. Gram Positives     A.  Cocci

         1)  Clusters

i. catalase + Staph sp

ii. coagulase + S. aureus

iii. coagulase - S. epidermidis

         2)  Pairs/chains -

                   i. catalase -  Strep sp.

B. Rods Cult Morphology Listeria

Small

Aero tumbling Diphtheroids Small Anaero pallisades Actinomyces Small Anaero Branching Propionibac Small Anaero clumps/pleo Lactobacillus Variable Both may chain Clostridium Large Anaero Spores Bacillus Large Aero Spores

III. Anaerobes1. Origin - gut, mouth, head/neck (50% of chronic sinusitis), lung, female gu tract

2. Cant grow unless culture specifically asked. 3% of bacteremia, <1% of cystitis

3. Bacteroides (B.frag GNR) (Peptostreptococus GPC) (P. Acnes GPR), Clostridium GPR)

7/2/09 PANI